OAND N-TYPING OF SHIGATOXIN-PRODUCING E. COLI IN CHILDREN WITH POST-DIARRHEAE HEMOLYTIC-UREMIC SYNDROME IN BELARUS
https://doi.org/10.51922/1818-426X.2025.1.97
Abstract
A quantitative PCR study was conducted using TaqMan Array Card on stool samples from 97 children in the acute phase of hemolytic uremic syndrome (HUS). Diarrheagenic E. coli was detected in 45 children, in 88.9 % (40/45) of cases it was shigatoxin-producing (STEC). Shiga toxin type 2 was detected in 67.5 % (27/40) of STEC strains, and both Shiga toxins: types 1 and 2 were detected in 32.5 % (13/40). Serotyping of 40 STEC strains using PCR allowed to establish the O serogroup for 77.5 % (31/40) of them. The most frequently encountered serogroups were: O157 – in 32.5 % (13/40) of the samples, O111 – in 17.5 % (7/40) and O145 – in 12.5 % (5/40). Three more O-serogroups (O55, O104 and O26) accounted for 15.0 % of the samples. Typing of the H antigen performed for 7 STEC strains using fliC gene sequencing showed that the H4 antigen was the most common. Analysis of the relationships between STEC O-serogroups and the severity of the course and outcome of HUS in a small group of patients showed that the groups of patients with O157 (13 people) and non-O157 (27 people) STEC-HUS practically did not differ in clinical and laboratory parameters, severity of the course and complications.
About the Authors
G. V. Semeiko
Научно-исследовательский институт гигиены, токсикологии, эпидемиологии, вирусологии и микробиологии ГУ «Республиканский центр гигиены, эпидемиологии и общественного здоровья»
Belarus
Belarus
V. L. Kolodkina
Научно-исследовательский институт гигиены, токсикологии, эпидемиологии, вирусологии и микробиологии ГУ «Республиканский центр гигиены, эпидемиологии и общественного здоровья»
Belarus
Belarus
S. V. Baiko
УО «Белорусский государственный медицинский университет»
Belarus
Belarus
M. D. Charadnichenka
УЗ «4-я городская детская клиническая больница»
Belarus
Belarus
E. O. Samoilovich
Научно-исследовательский институт гигиены, токсикологии, эпидемиологии, вирусологии и микробиологии ГУ «Республиканский центр гигиены, эпидемиологии и общественного здоровья»
Belarus
Belarus
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Review
For citations:
Semeiko G.V., Kolodkina V.L., Baiko S.V., Charadnichenka M.D., Samoilovich E.O. OAND N-TYPING OF SHIGATOXIN-PRODUCING E. COLI IN CHILDREN WITH POST-DIARRHEAE HEMOLYTIC-UREMIC SYNDROME IN BELARUS. Medical Journal. 2025;(1):97-103. (In Russ.) https://doi.org/10.51922/1818-426X.2025.1.97
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