Machine perfusion for decellularization of unsuitable for transplantation human liver

Currently, the only method that can replace all the liver functions is organ transplantation. Due to the donor organs shortage, various bioartificial liver models are being developed. Decellularization of a liver unsuitable for transplantation from a deceased donor is a challenging topic of research. Data on the technical features of the preparation of the liver for the process of decellularization are given. An algorithm for obtaining a cell-free matrix of the human liver and the process of decellularization using a device for dynamic conservation of donor organs with subsequent evaluation by electron microscopy are described.

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