EFFECT OF CELL THERAPY ON OUTCOMES OF FULMINANT ACUTE NECROTIZING PANCREATITIS IN RATS

Aim. To evaluate the effect of cell therapy on outcomes of the fulminant form of acute necrotizing pancreatitis in rats.
Material and methods. Fulminant acute necrotizing pancreatitis was induced by the introduction of a 0.3 ml of 10 % solution of non-ionic polyethylene glycol octylphenol ether detergent into the caudal part of the rat pancreas. To comparatively evaluate the effect of cell therapy on the outcomes of the fulminant form of acute necrotizing pancreatitis, 0.9 % NaCl, mesenchymal stromal cells (MSCs) and MSC-derived microvesicles (MSC MVs) were intravenously injected into experimental animals 6 hours after the onset of disease modeling. The survival function in the groups was assessed using the Kaplan-Meier method. Organs and tissues was performed from all surviving and dead animals. Histologic changes in pancreatic tissues and target organs were studied by staining preparations with hematoxylin and eosin.
Results. The animals of the control group developed a fulminant form of the disease, in which against the background of subtotal necrosis of the pancreas there was a systemic microcirculatory disorder with the formation of disseminated intravascular coagulation syndrome, signs of multiple organ damage and 100 % mortality without treatment. The mortality in the group where intravenous saline was used for treatment was 83.3 %. Early use of MSC MVs also contributed to increased survival of experimental animals, the mortality was 16.7 %. In the group where MSCs were intravenously injected for early treatment all animals survived and were removed from the experiment on the 7th day for pathomorphological examination.
Conclusion. Intravenous administration of cell therapy (MSCs and MSC MVs) 6 hours after the onset of disease modeling has a positive effect on the survival of experimental animals with fulminant acute necrotizing pancreatitis.

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