MYCOBACTERIUM FORTUITUM AS A TEST CULTURE FOR ACCELERATED DETERMINATION OF THE DISINFECTANTS EFFECTIVENESS AGAINST MYCOBACTERIA
https://doi.org/10.51922/1818-426X.2025.1.104
Abstract
In accordance with the regulatory documents governing the testing of anti-tuberculosis effect, tuberculocidal regimens for disinfectants should be developed only based on the reference strain M. terrae ATC C 15755 as a target, since non-pathogenic M. terrae has a higher resistance to disinfectants than pathogenic mycobacteria. However, the time frame for disinfectants effectiveness assessment on the M. terrae test culture is at least 3 weeks. The aim of the work: to select a test culture from among the reference strains of fastgrowing non-tuberculous mycobacteria comparable in resistance to M. terrae ATCC 15755 for the purpose of disinfectants effectiveness testing acceleration. The comparative study of M. terrae ATCC 157, M. fortuitum ATCC 6841, M. smegmatis ATCC 19420 and M. phlei ATCC 1175 by a quantitative suspension method revealed similar minimal effective concentrations for all six studied disinfectants from different chemical groups in relation to M. terrae and M. fortuitum. This indicates the possibility of the M. fortuitum reference strain usage for accelerated (within 4–5 days) determination of the disinfectants effectiveness in relation to mycobacteria.
About the Authors
G. A. Skorokhod
УО «Белорусский государственный университет»
Belarus
Belarus
E. I. Gudkova
ГУ «Республиканский центр гигиены, эпидемиологии
и общественного здоровья» (НИИ гигиены, токсикологии, эпидемиологии, вирусологии и микробиологии)
Belarus
Belarus
Zh. F. Tsirkunova
УО «Белорусский государственный университет»
Belarus
Belarus
I. N. Slabko
УО «Белорусский государственный университет»
Belarus
Belarus
N. N. Berdnik
УО «Белорусский государственный университет»
Belarus
Belarus
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Review
For citations:
Skorokhod G.A., Gudkova E.I., Tsirkunova Zh.F., Slabko I.N., Berdnik N.N. MYCOBACTERIUM FORTUITUM AS A TEST CULTURE FOR ACCELERATED DETERMINATION OF THE DISINFECTANTS EFFECTIVENESS AGAINST MYCOBACTERIA. Medical Journal. 2025;(1):104-107. (In Russ.) https://doi.org/10.51922/1818-426X.2025.1.104
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