Preview

Medical Journal

Advanced search

Approbation of methods for detecting bacteria of the STAPHYLOCOCCUS AUREUS species in the air environment of the premises of healthcare organizations in a model experiment

https://doi.org/10.51922/1818-426X.2022.4.60

Abstract

This article presents the results of testing methods for detecting bacteria of the Staphylococcus aureus species in the air environment of healthcare facilities in a model experiment in a closed laminar flow hood using typical strains of microorganisms and isolates isolated during air monitoring. The tests were carried out in the microbiology laboratory of Republican unitary enterprise “Scientific practical centre of hygiene” in the 1st quarter of 2021 – the 2nd quarter of 2021. It has been established that the optimal volume of air taken to monitor compliance with the hygienic standard for Staphylococcus aureus in 1 m3 is 500 dm3 per 2 cups. This amount of sampled air will allow the counting of colonies, taking into account their morphology.

About the Authors

A. I. Zhabrouskaya
Научно-практический центр гигиены
Belarus


O. A. Emeliyanova
Научно-практический центр гигиены
Belarus


N. V. Dudchik
Научно-практический центр гигиены
Belarus


References

1. Staphylococcal infection microbiology. Staphylococcus and staphylococcal infections. Basic research methods [Electronic resource]. – Access of mode: https://2s5.ru/diagnosis/stafilokokkovaya-infekciya-mikrobiologiya-stafilokokk-i-stafilokokkovye. – Access of date: 25.06.2022.

2. MicroBio: Staphylococcus aureus [Electronic resource]. – Access of mode: https://mibio.ru/contents.php?id=86. – Access of date: 25.06.2022.

3. Assessment of bioaerosol particle characteristics at different hospital wards and operating theaters: a case study in Tehran / F. Bolookat [et al.] // MethodsX. – 2018. – Vol. 5. – P. 1588–1596.

4. Characteristics of the microbiota of the air environment in the premises of health care institutions of various classes of cleanliness” / N. V. Dudchik [et al.] // Health and Environment: Sat. scientific tr. / Ministry of Health of the Republic of Belarus. Scientific-practical. hygiene center; under total ed. N. P. Zhukova; ch. ed. S. I. Sychik. – Minsk: RIVSH, 2019. – Issue. 29. – P. 7–12.

5. Instructions for use №. 002–0521 “Methods for detecting bacteria of the species Staphylococcus aureus in the air environment of premises of healthcare organizations” approved. deputy min. Ch. state dignity. doctor of the Republic of Belarus dated May 21, 2021.

6. Experimental modeling of aerosols of microorganisms producers in the air of the working area as a risk factor for the impact on the health of workers in biotechnological production / N. V. Dudchik [et al.] // Health risk analysis. – 2017. – № 3. – S. 127–134. – DOI: 10.21668/health.risk/2017.3.15.


Review

For citations:


Zhabrouskaya A.I., Emeliyanova O.A., Dudchik N.V. Approbation of methods for detecting bacteria of the STAPHYLOCOCCUS AUREUS species in the air environment of the premises of healthcare organizations in a model experiment. Medical Journal. 2022;(4):60-66. (In Russ.) https://doi.org/10.51922/1818-426X.2022.4.60

Views: 6


Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 License.


ISSN 1818-426X (Print)